Universitat Rovira i Virgili

Altres notícies

Darreres publicacions

Probing Site-Selective Conjugation Chemistries for the Construction of Homogeneous Synthetic Glycodendriproteins

Isidro Cobo, M. Isabel Matheu, Sergio Castillón, Benjamin G. Davis, Omar Boutureira

ChemBioChem 2022, e202200020

Abstract: Methods that site-selectively attach multivalent carbohydrate moieties to proteins can be used to generate homogeneous glycodendriproteins as synthetic functional mimics of glycoproteins. Here, we study aspects of the scope and limitations of some common bioconjugation techniques that can give access to well-defined glycodendriproteins. A diverse reactive platform was designed via use of thiol-Michael-type additions, thiol-ene reactions, and Cu(I)-mediated azide-alkyne cycloadditions from recombinant proteins containing the non-canonical amino acids dehydroalanine, homoallylglycine, homopropargylglycine, and azidohomoalanine

Compartir

  • Twitter
  • Google+
  • Facebook
  • Linkedin

Pujar